Sequence:

SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAI VQT LVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Molecular weight:

Dimer, 13.1/26.2 kDa (116/232 aa)

Recipe:

Freeze dry from a 0.2 μ m filtered solution containing 0.1% trifluoroacetic acid (TFA)

Protein purity:

≥ 95% determined by reduced and non reduced SDS-PAGE analysis

Endotoxins:

≤ 0.1 EU/μg

Biological activity:

Alkaline phosphatase activity induced in ATDC-5 cells ≤ 15 ng/mL

English description:

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic sub-family (BMP) and functions as a critical element involved in the culture and differentiation of stem cells. BMP-4 is integral in stem cell cultures and the differentiation of mesoderm and ectoderm from pluripotent stem cells (iPSCs and ESCs).